Authentic Aminos – Premium 5-Amino-1MQ Research Compound

What Is 5-Amino-1MQ?

Authentic aminos research begins with understanding the precise molecular tools available for metabolic study. 5-Amino-1MQ (5-amino-1-methylquinolinium) is a selective small-molecule inhibitor of nicotinamide N-methyltransferase (NNMT), an enzyme central to nicotinamide metabolism and cellular energy regulation . For researchers seeking authentic aminos compounds with verified purity and documented activity, 5-Amino-1MQ represents a well-characterized tool for investigating NAD+ biology, methylation pathways, and metabolic signaling.

Unlike broad-spectrum metabolic modulators, 5-Amino-1MQ acts through a defined intracellular enzyme target. According to Sigma-Aldrich’s product documentation, it functions as a substrate site-targeting, selective NNMT inhibitor with an IC50 of 1.2 µM, reducing 3T3-L1 lipogenesis (EC50 = 30 µM) and adipocyte 1-methylnicotinamide levels (EC50 = 2.3 µM) without affecting related methyltransferases or enzymes in the NAD+ salvage pathway . This selectivity makes 5-Amino-1MQ valuable for authentic aminos research designs requiring mechanism-specific intervention.

For laboratories prioritizing authentic aminos quality standards, Penguin Peptides Lab supplies 5-Amino-1MQ with third-party verification and batch-specific Certificates of Analysis.

Product Specifications

Research Applications

NNMT Inhibition and Enzyme Kinetics

Authentic aminos research involving 5-Amino-1MQ centers on its interaction with NNMT, an enzyme that catalyzes methyl group transfer from S-adenosyl-L-methionine (SAM) to nicotinamide, producing S-adenosyl-L-homocysteine and 1-methylnicotinamide . Research published through Diamond Light Source and AstraZeneca has characterized the mechanism and kinetics of NNMT turnover inhibitors, demonstrating that 5-Amino-1MQ acts through an on-target biotransformation mechanism combining substrate cell permeability with product inhibitor potency . For researchers requiring authentic aminos tools with documented enzymatic activity, this compound provides a validated framework for NNMT studies.

NAD+ Pathway and Cellular Metabolism

By inhibiting NNMT, 5-Amino-1MQ increases intracellular NAD+ levels and modulates the cellular SAM/SAH ratio . Springer’s publication on NAD+ in fatty liver disease notes that NNMT upregulation is associated with metabolic pathogenesis, making selective inhibitors valuable for investigating NAD+-dependent signaling pathways . Authentic aminos research applications include studying how NNMT inhibition affects mitochondrial function, ATP-related processes, and cellular adaptation under metabolic stress.

Metabolic Signaling and Lipogenesis

Preclinical research has demonstrated that 5-Amino-1MQ reduces lipogenesis in adipocytes and promotes weight loss in diet-induced obese murine models without affecting food intake . The compound’s effects on energy expenditure, adipose mass reduction, and plasma lipid profiles provide authentic aminos researchers with a defined tool for metabolic pathway investigation . These findings support further study of NNMT inhibition as a research strategy in metabolic disease models.

Comparative Pathway Studies

Because 5-Amino-1MQ targets a defined intracellular enzyme rather than receptor-based signaling, it enables researchers to isolate NNMT-specific effects from broader metabolic pathways . Laboratories seeking authentic aminos compounds for comparative studies can use 5-Amino-1MQ alongside other metabolic modulators to differentiate mechanism-specific outcomes.

Quality and Purity

Each batch of 5-Amino-1MQ undergoes rigorous quality control testing to ensure:

• ≥99% purity verified by HPLC

• Correct molecular weight confirmed by mass spectrometry

• Consistent compound content validated by analytical testing

Certificates of Analysis (COA) are available upon request, providing full transparency for research applications. For authentic aminos research, batch documentation ensures reproducibility across experimental protocols.

Frequently Asked Questions

What is 5-Amino-1MQ?

5-Amino-1MQ (5-amino-1-methylquinolinium) is a selective small-molecule inhibitor of nicotinamide N-methyltransferase (NNMT), an enzyme involved in nicotinamide metabolism and cellular energy regulation . It is studied in metabolic research for its effects on NAD+ biology and lipogenesis pathways.

What purity level can I expect?

Our 5-Amino-1MQ is provided at ≥99% purity, verified by HPLC. Batch-specific Certificates of Analysis document analytical results for authentic aminos research applications .

How should 5-Amino-1MQ be stored?

5-Amino-1MQ should be stored at -20°C for long-term stability. Lyophilised powder should be protected from light and moisture. After reconstitution, refrigerated storage at 2–8°C is recommended .

Is 5-Amino-1MQ FDA-approved?

No. 5-Amino-1MQ is not FDA-approved for any medical condition. It is supplied strictly for laboratory research use and is not intended for human or animal consumption .

What is the mechanism of action?

5-Amino-1MQ selectively inhibits NNMT by targeting the substrate binding site. This inhibition increases intracellular NAD+ levels, modulates SAM/SAH ratios, and reduces lipogenesis in adipocyte models . The compound has demonstrated in vivo efficacy in murine models of diet-induced obesity at 20 mg/kg/day subcutaneous dosing .

What dosage is available?

Our 5-Amino-1MQ is available in 5mg per vial, providing sufficient material for extended research protocols .

What research applications are supported?

5-Amino-1MQ supports research in NNMT inhibition, NAD+ pathway investigation, cellular metabolic signaling, lipogenesis studies, and comparative metabolic pathway analysis . All applications are limited to laboratory research.

References

• Sigma-Aldrich: 5-Amino-1-methylquinolinium iodide – Selective NNMT inhibitor characterization, IC50 data, and in vivo efficacy 

• BenchChem: 5-Amino-1MQ Documentation Hub – In vitro and in vivo performance data, DIO mouse model protocols 

• Springer: NAD+ in fatty liver disease – NNMT association with metabolic pathogenesis 

• Elite Research Labs: 5-Amino-1MQ 5mg Research Peptide – Product specifications, COA documentation, research applications 

• Superpower: 5-Amino-1MQ NNMT Inhibitor Research – Safety considerations, regulatory status, and research context 

• Diamond Light Source: NNMT turnover inhibitor mechanism – AstraZeneca research on NNMT inhibitor kinetics 

Conclusion

5-Amino-1MQ is a well-characterized selective NNMT inhibitor for researchers investigating NAD+ biology, cellular metabolism, and metabolic signaling pathways. When sourcing authentic aminos research compounds, prioritize suppliers offering third-party tested products with verified purity and transparent batch documentation.

For researchers requiring reliable authentic aminos compounds for metabolic studies, Penguin Peptides Lab provides 5-Amino-1MQ with Certificates of Analysis available upon request.

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